Sludge, according to a wikipedia entry, is the residual material produced after sewage treatment of wastewater. As disgusting as it sounds, it is important to know what it contains which may play a role in sewage treatment, recycling water as well as protecting our water bodies from pollution.
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| SLUDGE anyone? Image Source:http://memegenerator.net/instance/59561637 |
I didn't want to spoil appetites by putting the picture of sludge. So here is Dr. Evil's picture.
Anyway, in a new study in Proteomics, they studied the metaproteome of sludge from a treatment plant. The experiment used SDS-PAGE and isoelectric focusing. LC-MS/MS analysis was carried out on a Bruker iontrap and data searched using Mascot with the usual 1% FDR. They identified several proteins and interestingly, 61% of spectra were of bacterial origin and 37% were eukaryotic (human and mouse). A small percentage included viruses. Central carbon and nitrogen metabolic pathways were found to be enriched.
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