High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics, Nature Biotechnology

People doing phosphoproteomics  have been achieving amazing numbers these days, partly because of many innovations in the field. Speaking of innovations, here is a new paper from the Mann lab published in Nature Biotechnology. Here, they talk about a novel approach to do phosphoproteomics called EasyPhos, which basically uses TFE (2,2,2-Trifluoroethanol) buffer for digestion and eliminates  fractionation steps because the TiO2 enrichment happens in a 96-well  deep well plate. 

They then studied insulin signaling in mice using this technique. They studied this with 11 time points and 6 biological replicates/time point. They analyzed 91 liver tissue phosphoproteomes, quantified about 31,000 phosphopeptides and accurately identified over 25,000 distinct phosphorylation sites in the liver.

The sheer scale of this study is overwhelming..

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