A protein sample when subjected to tandem mass spectrometry generates MS/MS spectra which are signatures of ions generated by the fragmentation of peptides. These MS/MS spectra can be compared against protein databases to identify proteins using computational algorithms known as " search algorithms".
There are many popular search algorithms, both commercial and free, available for use.
1. Mascot - Paid, a free version with limited functionality is available on the website.
http://www.matrixscience.com/
2. SEQUEST - Paid, available through the distributor's website
http://www.thermoscientific.com/ecomm/servlet/productsdetail?productId=11961811&groupType=PRODUCT&searchType=0&storeId=11152&from=search
However, free implementations of the SEQUEST algorithm are available which will discussed in the future.
3. OMSSA- Free
http://pubchem.ncbi.nlm.nih.gov/omssa/
4. X! Tandem - Free
http://www.thegpm.org/tandem/index.html
There are many popular search algorithms, both commercial and free, available for use.
1. Mascot - Paid, a free version with limited functionality is available on the website.
http://www.matrixscience.com/
2. SEQUEST - Paid, available through the distributor's website
http://www.thermoscientific.com/ecomm/servlet/productsdetail?productId=11961811&groupType=PRODUCT&searchType=0&storeId=11152&from=search
However, free implementations of the SEQUEST algorithm are available which will discussed in the future.
3. OMSSA- Free
http://pubchem.ncbi.nlm.nih.gov/omssa/
4. X! Tandem - Free
http://www.thegpm.org/tandem/index.html
No comments:
Post a Comment
Please provide your inputs